ISSN 2312-3672
Kurmyshkina O., Kovchur P., Volkova T. EXPERIMENTAL APPROACHES TO DERIVING PRIMARY TUMOR CELL CULTURES FROM CERVICAL CANCER BIOPSIES: A COMPARATIVE ANALYSIS OF PUBLISHED DATA AND OWN EXPERIENCE // Journal of Biomedical Technologies. 2017. № 1. P. 9‒24. DOI: 10.15393/j6.art.2017.3763
Issue № 1 (2017)
Methodical guidelines
EXPERIMENTAL APPROACHES TO DERIVING PRIMARY TUMOR CELL CULTURES FROM CERVICAL CANCER BIOPSIES: A COMPARATIVE ANALYSIS OF PUBLISHED DATA AND OWN EXPERIENCE
| Kurmyshkina Olga V. | Petrozavodsk State University, 33 Lenin str., Petrozavodsk, Russia 185910, studioza@mail.ru |
| Kovchur Pavel I. | Petrozavodsk State University, 33 Lenin str., Petrozavodsk, Russia 185910, pkovchur@mail.ru |
| Volkova Tatyana O. | Petrozavodsk State University, 33 Lenin str., Petrozavodsk, Russia 185910, VolkovaTO@yandex.ru |
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cervical cancer epithelial cells primary cell culture differentiation morphology phenotype |
The article provides a detailed description of the procedure of converting primary tumor cells of invasive cervical cancer into a state of a transient in vitro culture, including the description of conditions for tissue dissociation and selection of appropriate cell culture medium. Morphological characteristics of the cell colonies obtained and the results of their phenotyping (based on analysis of cytokeratin expression) are presented. Our own observations are discussed in comparison with the previously published studies aimed at defining optimal conditions to culture primary cells of cervical neoplasia. |
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